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As of Jan 2022, the reference genome used is Homo_sapiens/NCBI/GRCh38.

Processing involves the following steps:

• Raw fastq files uploaded to Synapse by researcher in a folder with name format experiment_name_rnaseq_fastq_date . No white space should be present in the filenames (all filenames should have _ for whitespaces. While the naming convention is a best practices recommendation and not a strict rule, the exclusion of whitespaces is required.

• All experiment and sample related annotations need to be added on Synapse before processing can start. This is a required step so that a sample sheet can be generated to trigger the processing workflow

• The sample sheet should contain the following information in the following format (saved as a .csv file) (More information here) :

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